The above Topoisomerase MedChemExpress conclusions are consistent using the analysis ALK6 list findings on Arabidopsis
The above conclusions are consistent together with the analysis findings on Arabidopsis thaliana and rice. We also identified that exogenous BR spraying upregulates theanine synthesis genes, namely, TS, GS, and ADC and cold resistance-related genes, namely, CBF, ICE. It can be inferred that exogenous BR spraying elevated the theanine content material in tea leaves and enhanced cold resistance of tea plants. Theanine (L-Theanine) can be a distinctive totally free amino acid in tea and primary component of tea. Our findings are concordant to the investigation results of Li et al. (2018). The effect of exogenous BR spraying around the growth and development of tea leaves and also the signal transduction pathway of BR in tea leaves was revealed by transcriptome analysis. Our results showed that the tea leaves sprayed with BRs were substantially different, along with the upregulated genes had been mainly related to BR signal transduction, sucrose synthesis, chlorophyll synthesis, starch synthesis, flavonoid biosynthesis, cell division, theanine synthesis, and cold resistance. Furthermore, we also discovered that after spraying BR, the important genes for caffeine synthesis had been down-regulated. Our study lays the foundation for elucidating the molecular mechanism from the BR signal transduction pathway in tea leaves and its regulatory role around the development and improvement of tea plants.Supplementary InformationThe on the net version includes supplementary material obtainable at doi. org/10.1186/s12864-021-08179-9. More file 1. Acknowledgements We thank LetPub (www.letpub.com) for its linguistic assistance through the preparation of this manuscript. Authors’ contributions QJ designed and performed the experiments, analyzed the data, drafted the paper. ZW prepared figures and tables. YC, YL, and NT participated in the experiment and data analysis. SL, JH and ZL conceived in the investigation,By means of KEGG enrichment and annotation, the UGP, SPS, GPI, PFP and EP genes involved in sucrose synthesis; the GSA, HEMD, POR, CHLH, and COA genes connected to ginseng chlorophyll synthesis; the AGPase,Jin et al. BMC Genomics(2022) 23:Web page 12 ofparticipated in coordination, information evaluation and interpretation, drafted and reviewed the manuscript. All of the authors read and authorized the final manuscript. Funding This operate was financially supported by the National Organic Science Foundation of China (32172629, U19A2030, 31670689), Provincial All-natural Science Foundation of Hunan (2020JJ4358), and Special Project of Central Government Guiding Neighborhood Science and Technologies Improvement (2019XF5041). Availability of information and materials All the information supporting our findings are contained inside the manuscript. All raw transcriptome data reported in this write-up happen to be deposited in the Sequence Read Archive (SRA) under accession quantity PRJNA756445.9.10. 11.12.DeclarationsEthics approval and consent to participate The tea trees used in this study came from the Chang’an Tea Teaching and Experimental Base of Hunan Agricultural University, Changsha City, Hunan Province, China, and no material license was necessary. Our study is in complete compliance using the regulations of the program. This short article has obtained a license to make use of KEGG application from Kanehisa Labs [402]. Consent for publication Not applicable. Competing interests The authors have no conflict of interest to declare. Author information 1 Important Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China. two National Investigation Center of Engineering and Technologies for Utiliz.