Ommercial sources [Table 1]. PTE, CTE, XTL, PME, GCE, TDE (98 , purity) were
Ommercial sources [Table 1]. PTE, CTE, XTL, PME, GCE, TDE (98 , purity) were acquired from Shanghai Tauto Biotech Co., Ltd. ATP was obtained from Shenzhen Meihe Biotech Co., Ltd. CDE and DCE (98 , purity) have been purchased from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). THP, IMP and ISO were obtained from Nationwide Institute for the Control of Pharmaceutical and Biological Goods (Beijing, China).Equipments and Chromatographic ConditionsThe analysis was performed on a Waters 2695 Alliance HPLC technique (Waters Corp., Milford, MA, USA), equipped that has a quaternary solvent delivery technique, an internet degasser, an autosampler, a thermostated compartment and a 2996 photodiode array detection. Data collection and integration were achieved by EmpowerTM Application (Waters Corp., Milford, MA, USA). All analytes were separated on a LunaC18 (two) column (250.six mm, 5.0 , Phenomenex) plus a C18 guard column was made use of just before the analytical column. The separation was carried out with gradient elution method and mobile phase A (acetonitrile) and B (0.1 phosphoric acid water alternative, adjusted with triethylamine to pH 5.6) ratios linear changed as follows: 0 15 min, 23 A; 15 26 min, 23 40 A; 26 65 min, 40 65 A. The total run time was 65 min at a flow fee of 1 mLmin. The next HPLC parametersTable one: A summary with the MDM2 manufacturer examined YZP samplesSample no. T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 C1 C2 S1 S2 S3 D1 D2 Commercial manufacturer Tongrentang, Tianjin Tongrentang, Tianjin Tongrentang, Tianjin Longzhong, Hubei Longzhong, Hubei Banzhou Ttianlong, Guangxi Duanxintang Dayu, Shandong Duanxintang Dayu, Shandong Hebang Yangguang, Sichuan Hebang Yangguang, Sichuan Rongyu, Jiangsu Rongyu, Jiangsu Kangyuan, Jiangsu Kangyuan, Jiangsu Kangyuan, Jiangsu Longshen Rongfa, Gansu Longshen Rongfa, Gansu Manufacturing date 2012-04-11 2012-06-28 2013-03-08 2013-01-03 2013-03-03 2012-11-02 2012-01-18 2012-03-24 2012-12-24 2012-11-05 2012-06-12 2012-08-04 2012-03-09 2012-10-05 2011-12-15 2012-05-20 2012-04-MATERIALS AND METHODSChemicals and ReagentsHPLCgrade COX drug acetonitrile and methanol have been obtained from Honeywell (Muskegon, MI, USA). HPLCgradeYZP: Yuanhu Zhitong prescriptionPharmacognosy Magazine | January-March 2015 | Vol eleven | IssueZhang, et al.: Quantitative Determination of Energetic Components in Yuanhu Zhitong Prescriptionab Figure one: Chemical structures and chromatograms of twelve elements (protopine, PTE; -allocryptopine, ATP; coptisine, CTE; xanthotol, XTL; palmatine, PME; dehydrocorydaline, DCE; glaucine, GCE; tetrahydropalmatine, THP; tetrahydroberberine, TDE; imperatorin, IMP; corydaline, CDE; isoimperatorin, ISO) (a); representative chromatograms and ultraviolet spectrogram for determination of twelve components in four dosage varieties of YZP (b).had been employed for your evaluation: Column temperature, thirty ; injection volume, 20 , and the detection wavelength, 280 nm.Regular stock solutionsRESULTS AND DISCUSSIONA Hypersil BDS C18 column (250 four.6 mm, 5.0 , elite) as well as a Phenomenex LunaC18 (2) column (250 four.6 mm, five.0 , Phenomenex) had been examined to optimize the chromatographic affliction. The resolution of Phenomenex LunaC18 (two) column was proved far better than that of Hypersil BDS C18 column. On account of the key elements of YZP are alkaloids, unique mobile phases with buffer have been attempted, for example methanol ater (0.1 phosphoric acid water solution, adjusted with triethylamine to pH four, pH four.5, pH 5, pH 5.5, pH 6), and acetonitrile ater (0.1 phosphoric acid water option, adjusted with t.