C one, was t tively assigned to hexoside of hydroxybenzoic acid [M – H]- m/z 137.Nutrients 2022, 14, 2287 ten of3.three. The Analysis of Time-Dependent Adjustments of Metabolites soon after Gut Microbiota TreatmenThe kinetic changes of metabolites of CM extract were registered after 3.three. The Evaluation of Time-Dependent Adjustments of Metabolites immediately after Gut Microbiota Remedy the treatmof crude extract with FS metabolites of CM extract had been registered immediately after the treatment in neg for 0 h, 1 h, 3 h, six h, and 24 h. Total ion chromatograms The kinetic changes of ESI mode had been presented h, 1 h, three 4, whereas UV Total ion chromatograms in of crude extract with FS for 0in Figure h, six h, and 24 h. chromatograms were attached as plementary Components (Figures S1 five). Generally, the metabolites originating unfavorable ESI mode have been presented in Figure 4, whereas UV chromatograms were attached from ki as Supplementary Supplies (Figures S1 five). Inphenolicthe metabolites originating from and f analytes have been classified mostly as common, acids, hydrolyzable tannins, kinetic analytes possess the information around the key and fragmentary ions are provided for the m noids. In Table two, been classified primarily as phenolic acids, hydrolyzable tannins, and flavonoids. In Table 2, the information around the most important and fragmentary ions are provided for the significant compounds. Probably the most abundant signals inside the analytes from 0 h to 24 h have been derived compounds. Probably the most abundant signals inside the analytes from 0 h to 24 h were derived from aromadendrin derivatives. The signals tentatively assigned to pelargonidin aromadendrin derivatives.RITA Purity & Documentation The signals tentatively assigned to pelargonidin hexuronide,hexuro kaempferol hexoside, cornuside were had been digested successively kaempferol hexoside, and and cornusidedigested successively (Table 2).Glucose oxidase Metabolic Enzyme/Protease (Table 2).PMID:23415682 Figure Ion chromatograms of ethanolic-aqueous extract extract from mas (CM; (A)) immediately after the Figure 4. 4. Ion chromatograms of ethanolic-aqueousfrom fruits of C.fruits of C. mas (CM; (A)) afte remedy with FS for 0 h (B), 1 h (C), h (C), 3 6 h(D), and 24 h and 24 h (F). treatment with FS for 0 h (B), 1 3 h (D), h (E), 6 h (E), (F).Nutrients 2022, 14,11 ofTable two. MS spectrum information of compounds detected in fractions incubated with FS based on time. No. 1 2 3 4 5 6 7 eight 9 10 11 Retention Time [min] 29.7 31.two 34.9 41.3 45.7 47.2 49.0 67.0 68.1 81.two 85.four max [nm] 350 265, 310, 364 260, 300, 350 265, 310, 364 345 270, 310, 365 280 265, 310, 350 270, 320, 360 270, 320, 355 260, 310, 350 [M – H]- 449 287 449 287 447 433 541 745 595 391 297 MS2- 431, 355, 329, 287, 269 259 431, 287, 269 269, 243, 165 327, 284 287 379 630, 388. 257 549, 505, 462 345 279, 185 Assigned Compound aromadendrin hexoside aromadendrin isomer pelargonidin hexuronide aromadendrin isomer kaempferol hexoside aromadendrin cornuside unidentified unidentified unidentified unidentifiedThe evaluation of transformation of CM metabolites just after ex vivo FS digestion for 24 h showed mainly phenolic derivatives for instance gallic acid [m/z 169 [M – H]- (Rt = three.7 min)], hydroxybenzoic acid [m/z 137 [M – H]- (Rt = 14.1 min)], and protocatechuic acid [m/z 153 [M – H]- (Rt = 25.6 min)]. Protocatechuic acid was noted in additional analytes immediately after six and 24 h of incubation with FS. It’s supposed that protocatechuic acid could possibly originate in the transformation of anthocyanins [31]. Despite the peak of hydroxybenzoic acid found within the analytes treated with FS in t = 0 h, 1 h, and 3 h, it was tentatively identified determined by MS spectrum i.