Name :
Influenza A H7N9 (A/Anhui/1/2013) Matrix protein 1 / M1 Protein (His Tag)

Biological Activity :

Background :
The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.

Biological Activity :
Testing in progress

Expression Host :

Source :
E. coli

Tag :

Protein Accession No. :

NCBI Gene ID :

Synonyms :

Synonyms :
cholinergic receptor, muscarinic 1

Amino Acid Sequence :

Molecular Weight :
The recombinant Matrix protein 1 of Influenza A virus (A/Anhui/1/2013(H7N9)) comprises 258 amino acids and has a predicted molecular mass of 28.5 kDa. The apparent molecular mass of the protein is approximately 26-30 kDa in SDS-PAGE under reducing conditions.

Purity :
> 85 % as determined by SDS-PAGE

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
Please contact us for more information.

Protein Construction :
A DNA sequence encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) Matrix protein 1 (EPI439506) (Met1-Lys252) was expressed with a C-terminal polyhistidine tag.

Buffer Solution :
Lyophilized from sterile 20 mM tris, 0.5 mM EDTA, 5% glycerol, 50 mM NaCl, pH 7.6.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :

References & Citations :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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