Name :
HIV-1 gp140 Protein (group M, subtype CRF07_BC) (His Tag)
Biological Activity :
Background :
Biological Activity :
1.Immobilized Recombinant HIV-1 gp140 Protein (group M, subtype CRF07_BC) (His Tag) (Cat: 11677-V08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD4 Protein (Fc Tag) (Cat: 10400-H02H), the EC50 is 70-210 ng/mL.2.Loaded Recombinant Human CD4 Protein, hFc Tag (Cat. No. 10400-H02H) on ProA Biosensor, can bind HIV-1 gp140 Protein (group M, subtype CRF07_BC), His Tag (Cat. No. 11677-V08H) with an affinity constant of 82.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Expression Host :
HIV
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NCBI Gene ID :
Synonyms :
Synonyms :
CUB domain containing protein 1
Amino Acid Sequence :
Molecular Weight :
The recombinant HIV-1 gp140 consists of 694 amino acids after removal of the signal peptide and has a predicted molecular mass of 78.2 kDa. The apparent molecular mass of the recombinant protein is approximately 119 KDa in SDS-PAGE under reducing conditions.
Purity :
> 94 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the HIV-1 envelope glycoprotein gp160 extracellular domain (Val30-Tyr677), termed as gp140, was fused with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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