Name :
SARS-CoV-2 (2019-nCoV) Methyltransferase / ME-his Recombinant Protein

Biological Activity :

Background :
Coronavirus encodes the 2’-O-MTase (2’O Methyltransferase) that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2’O-methyltransferase (2’O-MTase) is highly conserved. The conserved 2’O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting the 2’O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral drug development which can against SARS-CoV-2,SARS-CoV, MERS-CoV or other RNA and DNA viruses.

Biological Activity :
Testing in progress

Expression Host :

Source :
E. coli

Tag :

Protein Accession No. :

NCBI Gene ID :

Synonyms :

Synonyms :

Amino Acid Sequence :

Molecular Weight :
The recombinant SARS-CoV-2 (2019-nCoV) Methyltransferase / ME consists of 299 amino acids and predicts a molecular mass of 33.46 kDa.

Purity :
> 85 % as determined by SDS-PAGE.

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
Please contact us for more information.

Protein Construction :
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Methyltransferase / ME (YP_009724389.1) (Ser6799-Asn7096) was expressed with a polyhistidine tag at the C-terminus.

Buffer Solution :
Supplied as sterile 10mM Tris 250mM NaCl, 50% Glycerol pH 7.4.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
Liquid. It is shipped out with blue ice.

Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Synonyms :

References & Citations :
Wang Y, et al., Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis.J Virol. 2015 Yu Chen, et al., Biochemical and Structural Insights into the Mechanisms of SARS Coronavirus RNA Ribose 2′-O-Methylation by nsp16/nsp10 Protein Complex.PLOS Pathogens.2011Mickaël Bouvet, et al.,Coronavirus Nsp10: a Critical Co-Factor for Activation of Multiple Replicative Enzymes.JBC.2014

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites:
Popular product recommendations:
PLD4 Protein
IL-12R beta 2 Protein
Popular categories:
Ubiquitin-Specific Peptidase 40
Complement Component 1