Name :
Recombinant Human ARF3 Protein (His Tag)
Biological Activity :
Background :
ARF3, also known as ADP-ribosylation factor 3, belongs to the RAS superfamily. Members of this family include ARF1, ARF2, ARF3, ARF4, ARF5 and ARF6. ARF3 gene is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin subunit, an ADP-ribosyltransferase. It is involved in protein trafficking and may modulate vesicle budding and uncoating within the Golgi apparatus.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P61204
NCBI Gene ID :
Synonyms :
Synonyms :
ADP-ribosylation factor 3
Amino Acid Sequence :
Molecular Weight :
The recombinant human ARF3 consists of 196 amino acids and predicts a molecular mass of 22.4 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 85 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human ARF3 (P61204) (Met1-Lys181) was expressed with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Hirai M. et al., 1997, Genomics. 34 (2): 263-5. Kanoh H. et al., 1997, J Biol Chem. 272 (9): 5421-9. Boman. et al., 2002, Mol Biol Cell. 13 (9): 3078-95.
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