Name :
Recombinant Human DPP3 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Dipeptidyl peptidase 3 (DPP3) is a zinc-dependent peptidase distributed within the cytoplasm which cleaves dipeptides sequentially from the N-terminus of bioactive peptide substrates 4+ amino acid residues in length. DPP3 adopts a bi-lobed structure and features HEXXGH and EEXRAE/D motifs which coordinate the Zn2+ ion in its active site. DPP3’s gene is located on chromosome 11q12-13.113. DPP3 regulates the transformation of enkephalin, opioid peptide, terminal protein, and cell signaling. Deficiency results in ROS formation in the kidneys via elevated Angiotensin II (DPP3’s most prominent reported substrate), III, IV, and ANG (1-5). DPP3 is linked to the development of cardiovascular diseases through RAS, oxidative stress, and inflammation pathways. Depletion was associated with the upregulation of proapoptotic proteins SMAC and p53, making it a potential target in tumor therapy.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
NCBI Gene ID :
Synonyms :
Synonyms :
dipeptidyl-peptidase 3
Amino Acid Sequence :
Molecular Weight :
The recombinant Human DPP3 consisting of 745 amino acids and has a calculated molecular mass of 83.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 131.1 kDa protein in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the Human DPP3 (Q9NY33) (Ala2-Ala737) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 50mM Tris, 150mM NaCl, 10% Glycerol, 0.02% (w/v) Brij-35, pH 9.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
DPPIII Protein, Human DPP3 背景信息 Dipeptidyl peptidase 3 (DPP3) is a zinc-dependent peptidase distributed within the cytoplasm which cleaves dipeptides sequentially from the N-terminus of bioactive peptide substrates 4+ amino acid residues in length. DPP3 adopts a bi-lobed structure and features HEXXGH and EEXRAE/D motifs which coordinate the Zn2+ ion in its active site. DPP3’s gene is located on chromosome 11q12-13.113. DPP3 regulates the transformation of enkephalin, opioid peptide, terminal protein, and cell signaling. Deficiency results in ROS formation in the kidneys via elevated Angiotensin II (DPP3’s most prominent reported substrate), III, IV, and ANG (1-5). DPP3 is linked to the development of cardiovascular diseases through RAS, oxidative stress, and inflammation pathways. Depletion was associated with the upregulation of proapoptotic proteins SMAC and p53, making it a potential target in tumor therapy.
References & Citations :
1. Ye P, et al. DPP3: From biomarker to therapeutic target of cardiovascular diseases. Front Cardiovasc Med. 2022, doi: 10.3389/fcvm.2022.9740352. Malovan, G., et al. , The emerging role of dipeptidyl peptidase 3 in pathophysiology. FEBS J. 2023, doi: 10.1111/febs.164293. Tong, Y., et al. DPP3/CDK1 contributes to the progression of colorectal cancer through regulating cell proliferation, cell apoptosis, and cell migration. Cell Death Dis. 2021, doi: 10.1038/s41419-021-03796-44. Liu, J., et al. DPP3 expression promotes cell proliferation and migration in vitro and tumour growth in vivo, which is associated with poor prognosis of oesophageal carcinoma. Oncology Reports. 2023, doi: 10.3892/or.2022.8446
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