Name :
Recombinant Human GLT25D2 Protein (His Tag)
Biological Activity :
Background :
Glycosyl transferase 25 domain 2 (GLT25D2) is a glucosyltransferase enzyme expressed only at low levels in the nervous system. Glycosyltransferases are enzymes that act as a catalyst for the transfer of a monosaccharide unit from an activated nucleotide sugar (also known as the "glycosyl donor") to a glycosyl acceptor molecule, usually an alcohol. Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glt25D2 enzyme showed a strong galactosyltransferase activity toward various types of collagen and toward the serum mannose-binding lectin MBL which contains a collagen domain.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
Q8IYK4
NCBI Gene ID :
Synonyms :
Synonyms :
collagen beta(1-O)galactosyltransferase 2
Amino Acid Sequence :
Molecular Weight :
The recombinant human GLT25D2 consists of 632 amino acids and predicts a molecular mass of 73.8 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 85 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human GLT25D2 (Q8IYK4) (Met 1-Ser 622) was fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
C1orf17 Protein, Human; GLT25D2 Protein, Human; RP11-498P10.2 Protein, Human GLT25D2 背景信息 Glycosyl transferase 25 domain 2 (GLT25D2) is a glucosyltransferase enzyme expressed only at low levels in the nervous system. Glycosyltransferases are enzymes that act as a catalyst for the transfer of a monosaccharide unit from an activated nucleotide sugar (also known as the “glycosyl donor”) to a glycosyl acceptor molecule, usually an alcohol. Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glt25D2 enzyme showed a strong galactosyltransferase activity toward various types of collagen and toward the serum mannose-binding lectin MBL which contains a collagen domain.
References & Citations :
Schegg B, et al. (2009) Core glycosylation of collagen is initiated by two beta (1-O) galactosyltransferases. Mol Cell Biol. 29 (4): 943-52.Sricholpech M, et al. (2011) Lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture. J Biol Chem. 286 (11): 8846-56.O’Connor DH, et al. (2009) Reverse engineering the mouse brain. Nature. 461 (7266): 923-9.
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