The had been very equivalent. present responses Tetraethylammonium Membrane Transporter/Ion Channel determined by SECCM were fairly comparable.Figure 7. SECCM characterization from the CNTNEE. (a) Illustration in the SECCM speak to location, where the CNTNEE could be the Figure 7. SECCM characterization of your CNTNEE. (a) Illustration of your SECCM contact area, where the CNTNEE will be the functioning electrode and an Ag/AgCl wire inserted inside the micropipette is definitely the QRCE. (b) Sigmoidal voltammogram collected operating electrode and an Ag/AgCl wire inserted in the micropipette will be the QRCE. (b) Sigmoidal voltammogram collected employing SECCM. utilizing SECCM.3.four. Lead Detection in Acetate Buffer 3.4. LeadCNTNEEin Acetate Buffer detection of Pb2 in 0.1 M acetate buffer (pH 4.five, 40 mL) The Detection was applied for the usingThe CNTNEE was applied tovoltammetry (SWASV). The deposition buffer (pH dep) squarewave anodic stripping the detection of Pb2 in 0.1 M acetate possible (E4.five, wasmL) working with squarewave anodic(tdep) was 300 s. Right after the deposition of Pbdeposition po40 two.0 V, and also the deposition time stripping voltammetry (SWASV). The two on the CNTNEE, it was stripped off within the potential window time (tdep ) was V with Right after the deposition tential (Edep ) was 2.0 V, as well as the deposition of 2.0 V to 0.three 300 s. a possible step (Estep) of 0.010 V, an amplitude ofit was stripped off within the of 30 Hz. The SWASV2.0 V to 0.three V of Pb2 around the CNTNEE, 0.05 V, in addition to a frequency potential window of response in the CNTNEE improved as(Estep ) of 0.010 V, of Pb2 in acetate bufferV, plus a frequencyas shown using a potential step the Phenmedipham Autophagy concentration an amplitude of 0.05 (pH 4.five) enhanced, of 30 Hz. in Figure 8a, as well as a linear rangeCNTNEE improved because the concentration of Pb2 in acetate The SWASV response of the of 105 ppb Pb2 was observed. A calibration curve was constructed by plotting the peak height against Pb2 concentration (Figure of 105 ppb Pb2 was buffer (pH four.5) elevated, as shown in Figure 8a, as well as a linear range 8b). observed. A calibration curve was constructed by plotting the peak height against Pb2 A = 0.065 0.001 ppb 0.555 0.017 (6) concentration (Figure 8b). R = C (ppb i p = (0.065 0.001)0.999 ) (0.555 0.017)R2 = 0.999 (6)of 0.010 V, an amplitude of 0.05 V, along with a frequency of 30 Hz. The SWASV response in the CNTNEE improved as the concentration of Pb2 in acetate buffer (pH 4.five) elevated, as shown in Figure 8a, as well as a linear range of 105 ppb Pb2 was observed. A calibration curve was constructed by plotting the peak height against Pb2 concentration (Figure 8b).Appl. Sci. 2021, 11,A = 0.0.ppb0.0.ten of 13 (6)R = 0.Figure eight. Lead detection applying the CNTNEE. (a) SWASV response in 0.1 M acetate buffer (pH 4.5) with varying concentraFigure eight. Lead detection making use of the CNTNEE. (a) SWASV response in 0.1 M acetate buffer (pH 4.five) with varying concentrations Pb2 (0, ten, 15, 20, 25, 30, and 35 ppb). (b) Calibration curve for Pb2detection (n ==3). tions Pb2 (0, 10, 15, 20, 25, 30, and 35 ppb). (b) Calibration curve for Pb2 detection (n 3).The limit of detection (LOD) was determined to be 0.57 ppb, utilizing LOD = three a /b, applying The limit of detection (LOD) was determined = 3S / , where Sa would be the standard deviation of on the blank solution, as well as the slopeslope with the caliis the normal deviation the blank remedy, and b is will be the on the calibration exactly where bration curve. The relative standard deviation (RSD) forreproducibility with the CNTNEE curve. The relative typical deviation (RSD) for the the reproducibility of the CNTelectrodes was 18.three , whilst the RSD for.